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15th June 2021  Product update: rapidvirology staff writer

New Highly-Sensitive NGS Panel for New and Known SARS-CoV-2 Variants


Thermo Fisher Scientific has introduced the Ion AmpliSeq SARS-CoV-2 Insight Research Assay to improve SARS-CoV-2 surveillance. The assay is designed to enable early identification of new and known variants from samples that have lower viral loads. By sequencing more than 99 percent of the SARS-CoV-2 genome, the assay covers all potential serotypes.

The new Ion AmpliSeq SARS-CoV-2 Insight Research Assay broadens and improves local, regional and national surveillance efforts to discover emerging SARS-CoV-2 variants through highly sensitive next-generation sequencing (NGS). Combined with Thermo Fisher's NGS technology, the panel enables researchers to obtain epidemiological insights with rapid turn-around time, workflow automation, seamless informatics, and data uploading to public SARS-CoV-2 data repositories.

"RNA viruses such as SARS-CoV-2 mutate at high rates in response to selective environmental pressures, requiring labs and vaccine developers to monitor mutations diligently," said Andy Felton, vice president of clinical next-generation sequencing at Thermo Fisher. "Higher sensitivity next-generation sequencing assays are needed to identify mutations earlier in both symptomatic and asymptomatic individuals to help public health officials and labs globally track the spread of new and known variants for public health decisions and guidance locally."

Optimized to run on the Ion GeneStudio S5 System,* the assay includes 237 amplicons specific to SARS-CoV-2, with the addition of variant-tolerant primers for emerging variants. The increased sensitivity of this assay will empower researchers to sequence the complete genome and discover new variants from a variety of sample types, including nasopharyngeal and shallow nasal swabs, saliva, and others, and with cycle threshold (Ct) values higher than 28 or as low as 50 viral copies.

"Our lab has been sequencing positive COVID-19 samples since the fall and recently announced a partnership with the Centers for Disease Prevention (CDC) to enhance national surveillance of known and emerging variants," said Josh Arant, chief operating officer of MAKO Medical Labs. "Supporting the increased scale of our sequencing program, the new, highly sensitive assay allows us to quickly report back valuable insights on SARS-CoV-2 variants to inform public health professionals across the country."

"As one of the labs currently sequencing positive SARS-CoV-2 samples as part of the International Travel Testing Programme, we need precise and comprehensive sequencing technology that can help us understand which mutations are present in positive samples for effective monitoring of SARS-CoV-2 cross-border transmission," said Marco Loddo, Ph.D., BSc, co-founder, and scientific director at Oncologica. "The new assay offers a fast improvement on the sensitivity for lower titer samples, including those from asymptomatic individuals, and has been brilliant for the discovery of emerging variants of concern entering the UK."

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Date Published: 15th June 2021

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Note: This content has been edited by a rapidvirology.com staff writer for style and content.


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