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17th September 2021  Content supplied by: Creative Diagnostics

Evaluate Vaccination with Recombinant SARS-CoV-2 Antigens with Critical Mutations


Genetic variants of SARS-CoV-2 have been emerging and circulating around the world throughout the COVID-19 pandemic. The U.K. variant B.1.1.7, the Brazil variant P.1, the South Africa variant B.1.351, and the most recent Indian variant B.1.617 are of particular concern because of their high prevalence.

While the spike protein in each variant has several mutations, there are three that are of particular concern:

  • The N501Y mutation occurs in the receptor-binding domain of the spike protein, positioned to influence binding to the host ACE2 receptor.
  • The E484K mutation is found in both the South African strain (B.1.1.7) and the one that is now prevalent in Brazil (P.1). There is growing evidence that this mutation may diminish neutralizing effects of some antibodies.
  • K417 has mutated to N in the B.1.351 lineage, while it changed to T (K417T) in the P1 lineage identified in Brazil.

Creative Diagnostics is going full steam ahead on developing a collection of recombinant antigens for these variants, including Spike proteins and Nucleocapsid proteins, covering critical mutations such as K417N/T, E484K, N501Y, and D614G on spike protein and R203G, G204R, and P13L on Nucleocapsid protein. The reagents can be used to evaluate the efficacy of the antibodies and vaccination.

The mutations in these strains also occur in the nucleocapsid protein, which is commonly used as the biomarker in rapid antigen tests. It’s critical to assess whether the current commercial antigen tests can detect the mutated N proteins with the same sensitivity and specificity as their WT counterpart.

Click here for the full list of recombinant antigens or use the 'Request Information' button provided below for more details. 


    

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Date Published: 17th September 2021

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